Liquid nitrogen in the 160-liter dewar is typically under pressure. The pressure is used to force liquid nitrogen from the dewar when the withdraw valve is opened. Never open the dewar's liquid withdraw value without first securely attaching the supply hose. The discharge end of the supply hose should be placed into one of the lab's small transfer dewars before the valve is opened. The withdraw valve should be opened slowly because the pressure in the dewar may be high and overly rapid discharge of liquid nitrogen could cause splashing and severe frostbite burns.
When liquid nitrogen is being transfered into a transfer dewar there will be rapid boil-off of the liquid nitrogen and typically a plume of fog coming out of the top of the transfer dewar. This fog is very cold - below freezing at the mouth of the transfer dewar, and has a very high concentration of nitrogen. Be careful when picking up the transfer dewar immediately after it has been filled. The handle, which is near the top of the dewar, may be very cold. Use the thermal mitten to hold the dewar handle and avoid freezing your skin to the handle.